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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-mouse IL-17F Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-mouse IL-17F Antibody
3185025 25 µg $150.00       
3185030 100 µg $305.00       
Clone: 9D3.1C8
Isotype: Mouse IgG1, κ
Reactivity: Mouse
Immunogen: Mouse IL-17F-OVA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Application References:

1. Obregon-Henao A, et al. 2013. PLoS One. 8:80669. PubMed

Th17-polarized CD4+ T cells from

Th17-polarized CD4+ T cells from C57BL/6 mouse lymph node surface stained with CD4 (GK1.5) APC, then intracellularly stained with anti-mouse IL-17F (clone 9D3.1C8) Alexa Fluor® 488

Th17-polarized CD4+ T cells from

Th17-polarized CD4+ T cells from C57BL/6 mouse lymph node intracellularly stained with anti-mouse IL-17F (clone 9D3.1C8) Alexa Fluor® 488 and anti-mouse IL-17A (clone: TC11-18H10.1) APC



Description:

Interleukin 17F (IL-17F) is a 37 kD IL-17 family member.  The IL-17 family consists of six members including IL-17 (also called IL-17A), IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25), and IL-17F.  IL-17F shares the strongest similarity to IL-17A and forms a homodimer or heterodimer with IL-17A. It is produced by Th17 cells, mast cells, basophils, and epithelial cells.  IL-17F is an important regulator of inflammatory responses.  It is involved in host defense against mucoepithelial infection by Staphylococcus aureus and Citrobacter rodentium.  Over expression of IL-17F gene in the airway of mice is associated with airway neutrophilia, the induction of many cytokines, an increase in airway hyperreactivity, and mucus hyper-secretion. IL-17F is also involved in cancer immunity and autoimmune responses. IL-17F, like IL-17A, depends on IL-17R for its signaling in vitro and in vivo.  P38 MAPK, ERK1/2, Act1 (NF-KB activator protein 1), and TRAF6 are involved in IL-17F signaling.

Other Names: Interleukin-17F, ML-1,
Structure: Forms a 37 kD homodimer or heterodimer with IL-17A of the IL-17 family member.
Distribution: Th17 cells, mast cells, basophils, and epithelial cells.
Function: An important regulator of inflammatory responses, involved in cancer immunity and autoimmune responses as well.
Ligand Receptor: IL-17R (CD217)
Antigen References:

1. Dong C. 2008. Immunol. Rev. 226:80.
2. Kolls JK, et al. 2004. Immunity 21:467.
3. Aggarwal S, et al. 2002. J. Leukoc. Biol. 71:1.
4. Yang XO, et al. 2008. J. Exp. Med. 205:1063.
5. Ishigame H, et al. 2009. Immunity 30:108.
6. Hu Y, et al. 2010. J. Immunol. 184:4307.


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