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Safety Data Sheet (SDS) PE Anti-mouse CD452 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD45.2 Antibody
1149035 50 µg $55.00       
1149040 200 µg $170.00       
Clone: 104
Isotype: Mouse (SJL) IgG2a, κ
Reactivity: Mouse
Immunogen: B10.S mouse thymocytes and splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
6. Pascal V, et al. 2007. J. Immunol. 179:1751.
7. Burman AC, et al. 2007. Blood 110:1064.
8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
9. Phan TG, et al. 2007. Nature Immunol. 8:992.
10. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
11. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
12. Hirata T, et al. 2012. Int Immunol. 24:705. PubMed
13. Wen T, et al. 2013. PNAS. 110:6067. PubMed
14. Davila SJ, et al. 2014. J. Immunol. 192:4284. PubMed
15. Markey KA, et al. 2014. J Immunol. 192:5426. PubMed

BALB/c (solid line) and SJL

BALB/c (solid line) and SJL (broken line) splenocytes stained with 104 PE



Description:

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Other Names: Ly-5.2, LCA
Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice
Function: Phosphatase, T and B cell activation
Ligand Receptor: Galectin-1, CD2, CD3, CD4
Antigen References:

1. Suzuki K, et al. 2000. Immunity 13:691.