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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-rat CD103 AlphaE Integrin Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-rat CD103 (αE Integrin) Antibody
1627545 100 µg $180.00       
Clone: OX-62
Isotype: Mouse IgG1
Reactivity: Rat
Immunogen: Density gradient enriched lymph veiled cells from PVG rats
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen skin and intestine epithelium1,4, immunocytochemical staining of cytospin preparations4, and Western blot1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Brenan M, et al. 1992. J. Exp. Med. 175:1457. (IHC, WB)
2. Chen-Woan M, et al. 1995. J. Immunol. Methods 178:157.
3. Chen-Woan M, et al. 1996. J. Leuk. Biol. 59:196.
4. Brenan M and Rees DJ. 2000. Eur. J. Immunol. 30:1527. (IHC)

LOU rat intestinal intraepithelial lymphocytes

LOU rat intestinal intraepithelial lymphocytes were stained with CD3 FITC and CD103 (clone OX-62) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).





Description:

CD103, also known as Integrin αE or integrin αIEL, is a single pass transmembrane protein composed by two chains, heavy and light, linked by a disulfide bond. It is expressed in the cell surface as an αE/β7 heterodimer. CD103 mediates adhesion of intraepithelial T cells (IEL) to the epithelium. CD103 is expressed by IEL, effector and memory Tregs, and dendritic cells and their immediate precursors. E-cadherin is the ligand for CD103.

Other Names: Integrin αE, integrin E, integrin αIEL, integrin αOX-62, Itgae
Structure: Single pass transmembrane protein, composed by two chains, heavy and light, linked by a disulfide bond. Associates with integrin β7 to form the αE/β7 complex.
Distribution: Intraepithelial T lymphocytes, effector and memory Tregs, dendritic cells and their immediate precursors.
Function: Mediates adhesion of intraepithelial T cells (IEL) to epithelium.
Ligand Receptor: E-cadherin
Antigen References:

1. Varas A, et al. 1998. Blood 92:93.
2. Brissette-Storkus CS, et al. 2002. J. Leukoc. Biol. 71:941.
3. Hubert FX, et al. 2004. J. Immunol. 172:7485.
4. Tsuchihashi S, et al. 2006. J. Immunol. 176:616.
5. Lührmann A, et al. 2007. Immunol. Lett. 108:167.


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