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Safety Data Sheet (SDS) PerCP/Cy55 Anti-mouse CD11a/CD18 LFA-1 Antibody     Product Data Sheet (PDF)    
PerCP/Cy5.5 Anti-mouse CD11a/CD18 (LFA-1) Antibody
1305035 25 µg $95.00       
1305040 100 µg $270.00       
Clone: H155-78
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: TH-derived anti-I-Ak CTL clone A1 5.1.17
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

This clone is reported to have some effect on increasing LFA-1-mediated adhesion and does not block LFA-1 binding1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Pont S, et al. 1986. J. Immunol. 136:3750. (FC)
2. Dupont CD, et al. 2014. PLoS Pathog. 10:1004047. PubMed
3. Markey KA, et al. 2014. J Immunol. 192:5426. PubMed

C57BL/6 splenocytes were stained with

C57BL/6 splenocytes were stained with LFA-1 (clone H155-78) PerCP/Cy5.5 (filled histogram) or rat IgG1 PerCP/Cy5.5 isotype control (open histogram).



Description:

Lymphocyte function-associated antigen 1 or LFA-1 is a heterodimer composed of two members of the integrin family, αL (CD11a) and β2 (CD18).  LFA-1 is expressed primarily on lymphocytes, monocytes/macrophages, and granulocytes.  It is involved in cell adhesion and costimulation processes. The ligands for LFA-1 are ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).

Other Names: Lymphocyte function-associated antigen 1, ITGAL, ITGB2
Structure: Heterodimer composed of two members of the integrin family, &alphaL (CD11a) and β2 (CD18).
Distribution: Primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes
Function: Intercellular adhesion and costimulation
Ligand Receptor: ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), and ICAM-3 (CD242)
Antigen References:

1. Dugger KJ, et al. 2009. J. Neuroimmunol. 206:22.
2. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
3. Springer TA. 1994. Cell 76:301.
4. Lub M, et al. 1995. Immunol. Today 16:479.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
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