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Safety Data Sheet (SDS) Purified Anti-Cytochrome B-245 Heavy Chain Antibody     Product Data Sheet (PDF)    
Purified Anti-Cytochrome B-245 Heavy Chain Antibody
3850505 25 µg $100.00       
3850510 100 µg $220.00       
Clone: 54.1
Isotype: Mouse IgG1, κ
Reactivity: Human, Mouse
Immunogen: Partially purified human neutrophil flavocytochrome b
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2,
0.09% NaN3.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application:

WB - Quality tested
IF, ELISA, FC - Reported by the developer

Application Notes:

Clone 54.1 is useful in detecting both mouse and human gp91phox by WB.

Recommended Usage:

Each lot of this antibody is quality control tested by Western blotting. For Western blotting the suggested working dilution is 0.5 µg per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974. (WB FC)
2. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429. (FC)
3. Jun HS, et al. 2014. Blood. 123:2843. PubMed

Extracts of human neutrophils were

Extracts of human neutrophils were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-gp91phox antibody (clone 54.1). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.



Description:

The integral membrane protein flavocytochrome b (Cyt b), a heterodimer formed by a 91-kD glycoprotein (heavy chain) and a 22-kD polypeptide (light chain), is the catalytic core of the phagocyte NADPH oxidase and generates superoxide which plays a critical role in host defense. NADPH oxidase is a central well characterized component of the innate immune response against fungal and bacterial infections. Recent research also offers new insight into a novel role for this oxidase in the coordination of the adaptive immune response. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole.

Other Names: CYBB, gp91phox, cytochrome b-245 beta chain, Superoxide-generating NADPH oxidase heavy chain subunit
Structure: 570 amino acids with predicted molecular weight of 65 kD.
Distribution: Membrane, multi-pass membrane protein
Function: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
Interaction: Composed of a heavy chain (beta) and a light chain (alpha).
Antigen References:

1. Dinauer MC, et al. 1990. J. Clin. Invest. 86:1729.
2. Shimo-Nakanishi Y, et al. 2004. Atherosclerosis 175:109.
3. Nakano Y, et al. 2008. J. Clin. Invest. 118:1176.
4. Kou B, et al. 2009. Microvasc Res. 77:125.
5. Yazdanpanah B, et al. 2009. Nature 460:1159.
6. Purushothaman D, et al. 2009. J. Exp. Med. 206:1515.
7. Yan CS, et al. 2009. J. Immunol. 182:3696.