The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Moriyama Y, et al. 2008. Int. Immunol. 20:763. 2. Burghardt S, et al. 2013. J. Immunol. 191:5574. PubMed
C57BL/6 mouse CD4 and CD8 double-negative thymocytes were stained with CD24 PE and Notch1 (HMN1-12) APC (top) or Armenian hamster IgG APC isotype control (bottom).
The Notch receptors are highly conserved from invertebrates to mammals. Notch 1 and Notch 2 exhibit the greatest structural similarity among the four mammalian Notch receptors. Notch 3 has a number of structural and functional differences. The binding of Notch 3 to its ligands results in the proteolysis of Notch and movement of intracellular portions of Notch into the nucleus. This translocation triggers a series of signaling processes. Notch 3 is primarily expressed in adult arterial smooth muscle cells. Notch 3 gene mutation can cause CADASIL, an inherited early stroke syndrome.
Neurogenic locus notch homolog protein 1
Transmembrane receptor. Heterodimer consisting of a C-terminal fragment and N-terminal fragment probably linked by disulphide bonds. Contains 5 ankyrin repeats, 29-36 EGF repeats, 3 Notch/Lin repeats.
Highly expressed in the brain, lung, and thymus (CD4-CD8- cells and CD4-CD8+ cells). Lower levels of expression in spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney, and heart.
Regulates broad spectrum of various cell lineage commitment processes, including myogenesis, neurogenesis, gliogenesis, and lymphocyte development.
Jag 1,2; Delta 1,2
1. Ehebauer ME, et al. 2006. Biochem. J. 392:13. 2. Shimizu K, et al. 2000. Mol. Cell. Biol. 20:18. 3. Tanigaki K, et al. 2007. Nature Immunol. 8:4514. 4. Kraman M, et al. 2005. FASEB J. 19:1311.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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