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Safety Data Sheet (SDS) APC Anti-mouse TCR Vgamma2 Antibody     Product Data Sheet (PDF)    
APC Anti-mouse TCR Vγ2 Antibody
1288535 25 µg $80.00       
1288540 100 µg $195.00       
Clone: UC3-10A6
Isotype: Armenian hamster IgG
Reactivity: Mouse
Immunogen: G8 mouse T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Dent AL, et al. 1990. Nature 343:714.
2. Kelly KA, et al. 1993. Int. Immunol. 5:331.
3. Sperling AI, et al. 1992. J. Immunol. 149:3200.
4. Sperling AI, et al. 1997. J. Immunol. 159:86.
5. Shibata K, et al. 2014. J. Immunol. 192:2210. PubMed
6. Dong P, et al. 2014. PLoS Pathog. 9:112964. PubMed

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with CD3 PE and TCR Vγ2 (clone UC3-10A6) APC (top) or Armenian hamster IgG APC isotype control (bottom).





Description:

T cell receptor (TCR) Vγ2 bearing T lymphocytes make up a significant proportion of γδ TCR cells in late fetal and adult peripheral lymphoid tissues. TCR γδ T cells may play a role in immunological surveillance for stress-induced self-antigens. The frequency of Vγ2 expression in different strains varied from 12% to 54% in the TCR γδ repertoire. Variations in the levels of Vγ2+ cells are not associated with MHC haplotype. High Vγ2 expression is influenced by the TCR-δ locus.  Expanding Vγ2+ TCRγδ cells in B6 mice overwhelmingly use a Vδ7+ δ chain except in the DBA/2 strain.

Other Names: Vγ2 T-cell receptor, T cell receptor gamma 2
Structure: A member of Ig superfamily, associated with TCR δ chain and CD3 complex.
Distribution: T cell subset in fetal and adult peripheral lymphoid tissues and thymus.
Function: Might play a role in a previously unrecognized form of immunological surveillance for stress-induced self-antigens.
Ligand Receptor: Antigens
Antigen References:

1. Allison JP, et al. 1991. Annu. Rev. Immunol. 9:679.
2. O’Brien RL, et al. 2000. J. Immunol. 165:6472.