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Safety Data Sheet (SDS) PE Anti-mouse H-2Kb Bound To SIINFEKL Antibody     Product Data Sheet (PDF)    
PE Anti-mouse H-2Kb Bound To SIINFEKL Antibody
1308015 25 µg $95.00       
1308020 100 µg $290.00       
Clone: 25-D1.16
Isotype: Mouse IgG1, κ
Reactivity: Mouse
Immunogen: SIINFEKL pulsed RMA-S cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The 25-D1.16 monoclonal antibody specifically reacts with ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb or H-2Kb bound with an irrelevant peptide.  Additional reported applications (for relevant formats) include: Western Blotting1,3, immunofluorescence microscopy2,3, immunohistochemical staining of frozen tissue sections3 , and inhibition of T cell response to H-2Kb-SIINFEKL in vitro.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Mareeva T, et al. 2010. J. Immunol. Methods 353:78. (WB)
2. Dolan BP, et al. 2010. J. Immunol. 184:1419. (IF)
3. Porgador A, et al. 1997. Immunity 6:715. (WB, IF, IHC)
4. Herve J, et al. 2013. J. Immunol. 190:3163. PubMed.

C57BL/6 mouse splenocytes were pulsed

C57BL/6 mouse splenocytes were pulsed with (filled histogram) or without (cyan open histogram) SIINFEKL for 2 hours and then stained with 25-D1.16 PE or mouse IgG1, κ PE isotype control (broken line open histogram).



Description:

This antibody has been proven to be very useful in tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo.

Other Names: MHC class I molecule Kb bound to the peptide SIINFEKL (Kb-SIINFEKL) H-2Kb, OVA agonist peptide (OVAp), OVA-Kb, OVA257-264 (SIINFEKL)-H-2kb, OVA257-264-H-2kb
Structure: OVA peptide257-264 and H-2Kb complex
Distribution: SIINFEKL bond H-2Kb bearing cells
Antigen References:

1. Mareeva T, et al. 2010. J. Immunol. Methods 353:78.
2. Mareeva T, et al. 2008. J. Biol. Chem. 283:29053.
3. Deng Y, et al. 1998. J. Immunol. 161:1677.