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Safety Data Sheet (SDS) APC Anti-mouse TCR Vgamma3 Antibody     Product Data Sheet (PDF)    
APC Anti-mouse TCR Vγ3 Antibody
1287525 25 µg $120.00       
1287530 100 µg $280.00       
Clone: 536
Isotype: Syrian hamster IgG
Reactivity: Mouse, Cross-Reactivity: No
Immunogen: AKR mouse dendritic epidermal cell clone 7-17
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported (for relevant formats) application include: immunoprecipitation1 and immunofluorescence2.

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California. This availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Havran WL, et al. 1989. P. Natl. Acad. Sci. USA 86:4189. (IP)
2. Havran WL, et al. 1999. J. Immunol. 142:1422. (IF)
3. Uchida Y, et al. 2011. J. Immunol. 186:6945. PubMed
4. Hu S, et al. 2013. J. Immunol. 190:3267. PubMed.

Mouse dendritic epidermal cell line

Mouse dendritic epidermal cell line 7-17 were stained with anti-mouse TCR Vγ3 (clone 536) APC (filled histogram) or hamster IgG APC isotype control (open histogram).



Description:

Vγ3+ T cells are the predominant γδ TCR-bearing cells in early fetal thymus.  The majority of CD3+ cells in 14-day fetal thymus express Vγ3.   Vγ3 is also expressed on Thy-1+ dendritic epidermal cells (Thy-1+ DECs).  Cells that express Vγ3 are not significantly detectable in adult lymphoid organs.  It has been reported that Lck and Fyn, two Src family kinases, are required for the development of Vγ3+ T cells.

Other Names: Vγ3 T-cell receptor, T cell receptor gamma 3
Structure: A member of Ig superfamily, heterodimerized with δ TCR chain and associated with CD3 complex.
Distribution: Dendritic epidermal T cells (DECs) and fetal murine thymocytes.
Function: Antigen recognition
Ligand Receptor: Antigens
Antigen References:

1. Allison JP, et al. 1991. Annu. Rev. Immunol. 9:679.
2. O’Brien RL, et al. 2000. J. Immunol. 165:6472.
3. Kelly KA, et al. 1993. Int Immunol. 5:331.
4. Moore TA, et al. 1996. J. Immunol. 157:2366.
5. Payer E, et al. 1991. J. Immunol. 146:2536.
6. Van Oers NSC, et al. 1996. Immunity 5:429.