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Safety Data Sheet (SDS) PE Anti-mouse CD355 CRTAM Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD355 (CRTAM) Antibody
1310025 25 µg $140.00       
1310030 100 µg $290.00       
Clone: 11-5/CRTAM
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: CRTAM extracellular region-human IgG Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for relevant formats) include:  Western blotting1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Arase N, et al. 2005. Int. Immunol. 17:1227. (WB)

C57BL/6 mouse splenocytes were stimulated

C57BL/6 mouse splenocytes were stimulated with immobilized TCR-β (clone H57-597) and then stained with CD8 Alexa Fluor® 647 and CD355 (clone 11-5/CRTAM) PE (top) or rat IgG2a, κ PE isotype control (bottom).





Description:

Class-I MHC-restricted T-cell associated molecule (CRTAM), designated as CD355, is an Ig domain like type I transmembrane receptor, which belongs to the nectin family. In the immune system, CRTAM is strictly expressed on activated NK cells and activated CD8-positive T cells. By binding its ligand Nectin-like molecule 2 (Necl2), CRTAM plays an important role in mediating cell adhesion and migration.

Other Names: Class I-restricted T cell-associated molecule
Structure: 75 kD homodimer, nectin family, Ig superfamily
Distribution: Activated NK cells and activated CD8-positive T cells
Function: Cell adhesion, migration
Ligand Receptor: Nectin-like molecule 2
Antigen References:

1. Takeuchi A, et al. 2009. J. Immunol. 183:4220.
2. Yeh JH, et al. 2008. Cell 132:846.
3. Arase N. 2005. Int. Immunol. 17:1227.
4. Boles KS, et al. 2005. Blood 106:779.
5. Galibert L, et al. 2005 J. Biol. Chem. 280:21955.
6. Kennedy J, et al. 2000 J. Leukoc. Biol. 67:725.