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Safety Data Sheet (SDS) Purified Anti-mouse CD283 TLR3 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD283 (TLR3) Antibody
1309510 100 µg $235.00       
Clone: 11F8
Isotype: Rat IgG2a
Reactivity: Mouse
Immunogen: Recombinant mouse TLR3 extracellular domain.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

ICFC - Quality tested
IHC - Reported in the literature

Application Notes:

Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue1.

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. McCartney S, et al. 2009. J. Exp. Med. 13:2967. (IHC)

C57BL/6 mouse splenocytes were surface

C57BL/6 mouse splenocytes were surface stained with CD4/CD19/Ly-6G/NK1.1 PerCP, CD11c APC and CD8 FITC, followed by intracellular staining with TLR3 (clone 11F8) PE (top) or rat IgG2a, κ PE isotype control (bottom). The data was analyzed by gating on CD4/CD19/Ly-6G/NK1.1 negative and CD8 positive cells.





Description:

Toll-like receptor 3 (TLR3), also known as CD283, is type I transmembrane protein that belongs to the TLR family. It forms a large horseshoe shape that contacts a neighboring horseshoe, forming a dimer of two horseshoes. It is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. TLR3 is expressed selectively on the cytoplasmic membrane and intracellularly in dendritic cells. It is also highly expressed in the placenta, pancreas, heart, liver, lung, and muscle. TLR3 is a pattern recognition receptor that participates in innate immune response to microbial pathogens by recognizing polyinosine-polycytidylic acid (Poly(I:C)) and dsRNA. Ligand binding by TLR3 induces receptor dimerization which results in inducing NF-κB activation (via TRIF-linked RIP1/TLR3 interactions) and cytokine production. TLR3 has been shown to interact with a number of proteins including MYD88, TRAF6, TRIAD3, MAP3K7, and TAB2.

Other Names: Toll like receptor 3
Structure: Toll-like receptor family member; type I membrane protein containing multiple leucine repeats and a interleukin-1 receptor like region in the extracellular domain. Predicted molecular weight approximately 116 kD.
Distribution: Selective expression in dendritic cells. Highly expressed in placenta and the pancreas; also expressed in heart, liver, lung and muscle.
Function: Pattern recognition receptor that participates in innate immune response to microbial pathogens. TLR3 ligands induce NF-κB (via TRIF-linked RIP1/TLR3 interactions) and cytokine production. TLR3 stimulation results in the production of cytokines.
Ligand Receptor: Polyinosine-polycytidylic acid (Poly(I:C)), dsRNA.
Interaction: MYD88, TRAF6, TRIAD3, MAP3K7, and TAB2.
Antigen References:

1. Alexopoulou L, et al. 2001. Nature 413:732.
2. Doyle SE, et al. 2002. Immunity 17:251.
3. Meylan E, et al. 2004. Nat Immunol. 5:503.
4. Muzio M, et al. 2000. J. Immunol. 164:5998.
5. Rock FL, et al. 1998. P. Natl. Acad. Sci. USA 95:588.