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Safety Data Sheet (SDS) Purified Anti-mouse CD200R3 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD200R3 Antibody
1311510 200 µg $180.00       
Clone: Ba160
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: Mouse primary basophils
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested

Application Notes:

Ba160 recognizes circulating and bone marrow basophils; it also recognizes a subset of mast cells in the peritoneal cavity and skin.

A single intravenous administration of 30 µg of LEAF Ba160 to mice induces a dramatic reduction of basophil number in peripheral blood from day 1 to day 10. *Tested in the development stage.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

C57BL/6 peripheral blood leukocytes were

C57BL/6 peripheral blood leukocytes were stained with FcεRIα APC and CD200R3 (clone Ba160) PE (top) or rat IgG2b, κ PE isotype control (bottom).





Description:

CD200R3, also known as CD200RLb and OX-2 Receptor 3, is a disulfide-linked dimmeric CD200R-like receptor which belongs to immunoglobulin superfamily.  Its positively charged aminio acid lysinein associates with ITAM- or YxxM motif-bearing adaptor molecules such as DAP12, DAP10, FcRγ, and CD3ζ. CD200R3 functions as an activating receptor to regulate IgE independent immune response.

Other Names: CD200R3, CD200RLb, OX-2 Receptor 3, CD200 receptor 3
Structure: Immunoglobulin superfamily; disulfide-linked dimmer with positively charged amino acids in transmembrane; molecular weight is about ~38 kD or ~74 kD (reduced or non-reduced condition, respectively).
Distribution: Basophils in peripheral blood, bone marrow, spleen: mast cells in peritoneal cavity and skin.
Function: Transduce stimulatory signals.
Interaction: DAP12, DAP10, FcRγ, or CD3ζ
Antigen References:

1. Voehringer D, et al. 2004. J. Biol. Chem. 52:54117.
2. Kojima T, et al. 2007. J. Immunol. 179:7093.
3. Sato K, et al. 2009. Blood 113:4780.