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Safety Data Sheet (SDS) APC Anti-mouse TIGIT Vstm3 Antibody     Product Data Sheet (PDF)    
APC Anti-mouse TIGIT (Vstm3) Antibody
1310525 25 µg $115.00       
1310530 100 µg $280.00       
Clone: 1G9
Isotype: Mouse IgG1, κ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Joller N, et al. 2010. J. Immunol. 186:1338.

Con A-stimulated C57BL/6 mouse splenocytes

Con A-stimulated C57BL/6 mouse splenocytes (3 days) were stained with CD3 PE and TIGIT (clone 1G9) APC (top) or mouse IgG1, κ APC isotype control (bottom).





Description:

T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as V-set and transmembrane domain-containing protein 3 (Vstm3), is a 26 kD, type I transmembrane protein and member of the CD28 family. TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. Its binding partners include CD155 (PVR) and CD112 (PVRL2). TIGIT is a negative regulator of NK and T cell activation. Engagement of TIGIT by dendritic cells results in their differentiation into a tolerogenic phenotype, with an increased secretion of IL-10 and a diminished production of IL-12. Mice deficient for TIGIT are more susceptible to autoimmune disease.

Other Names: VSIG9
Structure: Type I transmembrane protein, 26 kD, member of the CD28 family. Contains 1 Ig-like domain and 1 cytoplasmic ITIM motif.
Distribution: Activated T cells, follicular T helper cells, NK, memory T cells, Tregs.
Function: Negative regulator of T cell activation and proliferation. Induction of tolerogenic dendritic cells.
Ligand Receptor: CD155 (PVR) and CD112 (PVRL2).
Antigen References:

1. Levin SD, et al. 2011. Eur. J. Immunol. 41:902.
2. Yu X, et al. 2009. Nat. Immunol. 10:48.
3. Stanietsky N, et al. 2009. P. Natl. Acad. Sci. USA 106:17858.