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Safety Data Sheet (SDS) Purified Anti-mouse CD49a Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD49a Antibody
1313005 25 µg $60.00       
1313010 100 µg $180.00       
Clone: HMα1
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Mouse Neuroblastoma Cell Line C1300
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant format) include: inhibition of cell adhesion and cytokine production1,2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Miyake S, et al. 1994. Eur. J. Immunol. 24:2000. (FC, Block)
2. Tanaka T, et al. 1995. Int. Immunol. 7:1183. (Block)

C1300 (mouse adenocarcinoma cell line)

C1300 (mouse adenocarcinoma cell line) cells were stained with purified CD49a (clone HMα1, filled histogram) or Armenian hamster IgG (open histogram), followed by anti-hamster IgG PE.



Description:

CD49a is a 1179 aa, type I transmembrane glycoprotein also known as α1 integrin, VLA-1 α chain, or integrin α1. It associates antibody v042010 with CD29 (β1 integrin) to form the VLA-1 complex, a collagen IV and alminin-1 receptor that is expressed on activated T cells, smooth muscle cells, endothelial cells, neuronal cells, fibroblasts, and mesenchymal cells. CD49a is an adhesion molecule and is involved in the regulation of leukocyte migration, T cell proliferation, and cytokine production.

Other Names: Very late antigen 1 (VLA1), integrin alpha 1 (ITGA1)
Structure: 1179 aa, type I transmembrane glycoprotein
Distribution: Endothelial cells, fibroblasts, activated T cells
Function: Leukocyte adhesion and homing; costimulation
Ligand Receptor: Collagens, laminin
Antigen References:

1. Barczyk M, et al. 2010. Cell Tissue Res. 339:269.