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Safety Data Sheet (SDS) PE Anti-mouse CD49a Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD49a Antibody
1313015 25 µg $115.00       
1313020 100 µg $240.00       
Clone: HMα1
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Mouse Neuroblastoma Cell Line C1300
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant format) include: inhibition of cell adhesion and cytokine production1,2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Miyake S, et al. 1994. Eur. J. Immunol. 24:2000. (FC, Block)
2. Tanaka T, et al. 1995. Int. Immunol. 7:1183. (Block)
3. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
4. Burton BR, et al. 2014. Nat Commun. 5:4741. PubMed

C1300 (mouse neuroblastoma cell line)

C1300 (mouse neuroblastoma cell line) cells were stained with CD49a (clone HMα1) PE (filled histogram) or Armenian hamster IgG PE isotype control (open histogram).



Description:

CD49a is a 1179 aa, type I transmembrane glycoprotein also known as α1 integrin, VLA-1 α chain, or integrin α1. It associates antibody v042010 with CD29 (β1 integrin) to form the VLA-1 complex, a collagen IV and alminin-1 receptor that is expressed on activated T cells, smooth muscle cells, endothelial cells, neuronal cells, fibroblasts, and mesenchymal cells. CD49a is an adhesion molecule and is involved in the regulation of leukocyte migration, T cell proliferation, and cytokine production.

Other Names: Very late antigen 1 (VLA1), integrin alpha 1 (ITGA1)
Structure: 1179 aa, type I transmembrane glycoprotein
Distribution: Endothelial cells, fibroblasts, activated T cells
Function: Leukocyte adhesion and homing; costimulation
Ligand Receptor: Collagens, laminin
Antigen References:

1. Barczyk M, et al. 2010. Cell Tissue Res. 339:269.