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Safety Data Sheet (SDS) Purified Anti-mouse TCR Vgamma11 + Vgamma12 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse TCR Vγ1.1 + Vγ1.2 Antibody
1313510 100 µg $170.00       
Clone: 4B2.9
Isotype: Armenian hamster IgG
Reactivity: Mouse
Immunogen: TCR Vγ1.1/Vδ6 T3.13.1 hybridoma
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested

Application Notes:

mAb 4B2.9 specifically recognizes γ/δ T cells expressing Vγ1.1 and/or Vγ1.2 chains.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Pereira P and Boucontet L. 2004. J. Immunol. 173:3261. (FC)

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with TCR Vγ1.1 + Vγ1.2 (clone 4B2.9) PE (top) or Armenian hamster IgG PE isotype control (bottom).





Description:

In mice, the TCR g locus consists of seven Vg, four Jg and four Cg genes, which are differentially expressed through their lifespan. During fetal and early postnatal life, very few γ/δ T cells express TCR Vγ1.1 or Vγ1.2 chains. In adult mice, however, a major fraction of the γ/δ T cell repertoire comprise either TCR Vγ1.1 or Vγ1.2 chains. The Vγ1.1 gene rearranges almost exclusively with the Jg4-Cg4 genes, while the Vγ1.2 gene preferentially does with Jg1.

Other Names: Vγ1 and Vγ2 (Tonegawa nomenclature)
Distribution: Subset of γ/δ T cells
Function: Antigen recognition
Antigen References:

1. Garcia-Herrera A, et al. 2011. Am. J. Surg. Pathol. 35:1214.
2. Gutierrez A, et al. 2010. J. Clin. Oncol. 28:3816.
3. Zhang X, et al. 2009. Hematology 14:237.