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Safety Data Sheet (SDS) PE Anti-mouse CD59a Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD59a Antibody
1315515 25 µg $120.00       
1315520 100 µg $290.00       
Clone: mCD59.3
Isotype: Mouse IgG1, κ
Reactivity: Mouse
Immunogen: EL-4 cells transfected with murine CD59a
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: Western blotting of mouse erythrocytes1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Harris CL, et al. 2003. Immunology 109:117. (WB)

C57BL/6 mouse bone marrow cells

C57BL/6 mouse bone marrow cells were stained with CD59 (clone mCD59.3) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram). Data shown was gated on erythroid cell population.


CD59a, one of the two isoforms of CD59, is also known as membrane attack complex inhibition factor or MACIF. CD59a is a 18-25 kD GPI-anchored membrane glycoprotein of that protects cells from complement-mediated lysis by binding the complement factor C9, preventing its incorporation to the C5b-C8 complex, and impairing the formation of the membrane attack complex (MAC). CD59a is broadly expressed by different cell types, including endothelia, erythrocytes, and platelets.

Other Names: MACIF, 1F-5Ag, H19, HRF20, MIRL, P-18, Protectin
Structure: Glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein, 18-25 kD
Distribution: Different cell types, including endothelia, erythrocytes, and platelets
Function: Inhibits complement-mediated cell lysis by preventing the incorporation of factor C9 to the C5b-C8 complex, preventing the formation of the membrane attack complex (MAC)
Interaction: Complement factor C9
Antigen References:

1. Pu JJ and Brodsky RA. 2011. Clin. Transl. Sci. 4:219.
2. Yu Q, et al. 2010. Cell. Mol. Immunol. 7:334.
3. Kohli P and Levy BD. 2009. Br. J. Pharmacol. 158:960.