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Safety Data Sheet (SDS) Purified Anti-mouse CD159a NKG2AB6 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD159a (NKG2AB6) Antibody
1314010 500 µg $210.00       
Clone: 16A11
Isotype: Mouse IgG2b, κ
Reactivity: Mouse
Immunogen: C57BL/6 mouse CD94/NKG2A transfected CHO cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested

Application Notes:

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use licenses cannot be guaranteed from the University of California.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. McMahon CW, et al. 2002. J. Immunol. 169:1444. (FC)
2. Vance RE, et al. 2002. P. Natl. Acad. Sci. USA 99:868. (FC)

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with NK1.1 Alexa Fluor® 647 and purified CD159a (NKG2AB6) (clone 16A11) (top) or mouse IgG2b, κ isotype control (bottom), followed by anti-mouse IgG2b FITC.





Description:

CD159a, also known as NKG2A or KLRC1 (killer cell lectin-like receptor subfamily C, member 1), is a 43 kD type II transmembrane protein with extracellular C-type lectin domains.  It belongs to the killer cell lectin-like receptor family also known as the NKG2 family.  It is expressed on NK and NKT cells and activated CD8+ T cells.  NKG2A binds to non-classical MHC class I molecule Qa-1 and causes inhibition of NK cell-mediated target-cell lysis. 

Other Names: KLRC1, NKG2B
Structure: Type II transmembrane protein with extracelluar C-type lectin domains, complexed with CD94; 43 kD
Distribution: NK cells and NK T cells from C57BL/6 mouse
Function: Inhibitory activity via ITIMs
Ligand Receptor: Non-classical MHC-I molecule Qa-1
Antigen References:

1. Vance RE, et al. 1998. J. Exp. Med. 188:1841.
2. Lohwasser S, et al. 1999. Eur. J. Immunol. 29:755.
3. Sivakumar PV, et al. 1999. J. Immunol. 162:6976.
4. Silver ET, et al. 1999. Immunogenetics 49:727.
5. Vance RE, et al. 1999. J. Exp. Med. 190:1801.