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Safety Data Sheet (SDS) APC Anti-mouse CD41 Antibody     Product Data Sheet (PDF)    
APC Anti-mouse CD41 Antibody
1269565 25 µg $80.00       
1269570 100 µg $260.00       
Clone: MWReg30
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: Mouse platelets
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: depletion of platelets and functional assay in vivo.4,7

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Nieswandt B, et al. 1999. Blood 94:684.
2. Teeling JL, et al. 2001. Blood 98:1095.
3. Bertrand JY, et al. 2005. P. Natl. Acad. Sci. USA 102:134.
4. Nocito A, et al. 2007. Hepatology 45:369. (Deplete)
5. Sullivan BP, et al. 2010. Toxicol. Sci. 115:286. (Deplete) PubMed
6. van der Heyde HC, et al. 2005. Blood 105:1956. (FA)
7. Marjon KD, et al. 2009. J. Immunol. 182:1397. (Deplete)

Mouse platelets were stained with

Mouse platelets were stained with either anti-mouse CD41 (clone MWReg30) APC (filled histogram) or rat IgG1 APC isotype control (dashed histogram).



Description:

CD41, also known as integrin α2b and GPIIb, is a transmembrane glycoprotein that is expressed by platelets and megakaryocytes. It was reported that CD41 is also expressed on hematopoietic progenitors. CD41 associates with CD61 (integrin β3) to form complexes that interact with fibrinogen, fibronectin, von Willebrand factor, and thrombin. CD41 is required for platelet adhesion and aggregation. Defect of CD41 leads to disorders of coagulation.

Other Names: Fibrinogen receptor, gpIIb/IIIa, integrin alpha IIb
Structure: A transmembrane glycoprotein that is expressed by platelets and megakaryocytes
Distribution: Platelets, megakaryocytes, and hematopoietic progenitors
Function: Associate with CD61 (integrin β3) to form a complex which plays a major role in platelet adhesion and aggregation
Ligand Receptor: Fibrinogen, fibronectin, von Willebrand factor, thrombin
Antigen References:

1. Bakewell SJ, et al. 2003. P. Natl. Acad. Sci. USA 100:14205.
2. Phillips DR, et al. 1991. Cell. 65:359.