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Safety Data Sheet (SDS) PE Anti-mouse Trem-like 4 Treml4 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse Trem-like 4 (Treml4) Antibody
1316515 25 µg $120.00       
1316520 100 µg $290.00       
Clone: 16E5
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: Extracellular domain of recombinant mouse Trem-like 4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunofluorescent staining, immunoprecipitation, and Western blotting1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Hemmi H, et al. 2009. J. Immunol. 182:1278. (WB)

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with Treml4 (clone 16E5) PE (filled histogram) or rat IgG1, κ PE isotype control (open histogram). Data shown was from gated on CD8+CD11c+ cells.



Description:

Trem-like 4, a type I transmembrane protein and a member of the TREM family, consists of a single extracellular Ig-like domain of the V-type, a transmembrane domain, and a short cytoplasmic domain. Trem-like 4 is expressed on macrophages in the spleen, and dendritic cells, especially CD8+ dendritic cells. It is proposed to regulate the immune response based on its expression profile. It has been reported that B7-H3 is a receptor and that the soluble form of Treml4 has a binding affinity to late apoptotic or necrotic cells.

Other Names: Trem-l4, TREM like transcript 4, triggering receptor expressed on myeloid cells-like 4
Structure: Type I transmembrane protein; member of the TREM family
Distribution: CD8+ dendritic cells, dendritic cell subsets, macrophages in the spleen, myeloid cells
Function: Regulates innate immune response and homeostatis
Interaction: Associates with DAP12; binds late apoptotic cells/necrotic cells
Antigen References:

1. Ford JW and McVicar DW. 2009. Curr. Opin. Immunol. 21:38.
2. Hemmi H, et al. 2009. J. Immunol. 182:1278.