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Safety Data Sheet (SDS) APC Anti-rat CD161 Antibody     Product Data Sheet (PDF)    
APC Anti-rat CD161 Antibody
1628030 100 µg $190.00       
Clone: 3.2.3
Isotype: Mouse IgG1, κ
Reactivity: Rat
Immunogen: LEW rat splenic NK cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

3.2.3 recognizes a common epitope of NKR-1P1a (CD161a) and NKR-P1b (CD161b).

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Chambers W, et al. 1989. J. Exp. Med. 169:1373. (FC)

Lewis rat splenocytes were stained

Lewis rat splenocytes were stained with CD161 (clone 3.2.3) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).



Description:

CD161 is a 30 kD type II transmembrane C-type lectin, expressed as a homodimer. Rat NKR-P1 receptors are primarily expressed on NK cells, a subset of T cells, dendritic cells, and activated monocytes. There are three different types of NKR-P in rat, namely NKR-P1a, NKR-P1b, and NKR-P1c. NKR-P1a does not contain an ITIM structure and is an activating receptor, while NKR-P1b contains an ITIM and displays inhibitory function. LLT-1 (ligand lectin like transcript 1) is the ligand, while KLR (killer cell lectin like) functions as a receptor.

Other Names: NKR-P1, CD161a/CD161b, NKR-P1a/KLRB1a
Structure: Type II transmembrane C-type lectin, 30 kD.
Distribution: NK cells, subset of T cells, dendritic cells, activated monocytes.
Function: NKR-P1a is a NK cell-activating receptor; NKR-P1b is a NK cell-inhibitory receptor.
Ligand Receptor: LLT1-ligand lectin like transcript 1; KLR-killer cell lectin like receptor.
Antigen References:

1. Chambers WH, et al. 1989. J. Exp. Med. 169:1373.
2. Brink M, et al. 1990. J. Exp. Med. 171:197.
3. Li J, et al. 2003. Int. Immunol. 15:411.