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Safety Data Sheet (SDS) Purified Anti-mouse CD370 CLEC9A DNGR1 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD370 (CLEC9A, DNGR1) Antibody
1317510 100 µg $180.00       
Clone: 7H11
Isotype: Rat IgG1, κ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
ELISA, IF, IP, WB - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: ELISA2, immunofluorescence microcopy1, immunoprecipitation1, and Western blotting1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.

Application References:

1. Sancho D, et al. 2008. J. Clin. Invest. 118:2098. (IF, IP, WB)
2. Joffre OP, et al. 2010. Eur. J. Immunol. 40:1255. (ELISA)



Description:

CD370 (CLEC9A), also known as DNGR1, is a 264 amino acid (aa) type II transmembrane glycoprotein which belongs to the C-type lectin superfamily. It contains a 208 aa extracellular region with a single carbohydrate recognition domain (CRD) and a cytoplasmic segment (35 aa) bearing an immunoreceptor tyrosine-based activation motif (ITAM).  In mice, CLEC9A is strictly expressed on CD8a-positive dendritic cells and plasmacytoid dendritic cells. It recognizes a ubiquitous preformed acid-labile protein associated ligand(s) that is exposed on necrotic cells. CLEC9A ligation is necessary for efficient cross-presentation of dead-cell-associated antigen by recruitment and activation of the tyrosine kinase Syk.

Other Names: DNGR-1, C-type lectin domain family 9 member A, CD370
Structure: Type II transmembrane glycoprotein, consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM-like motif, a 21 aa transmembrane segment, and a 185 extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD)
Distribution: Strictly expressed on CD8α+ dendritic cells, migratory CD103+ dendritic cells, and plasmacytoid dendritic cells
Function: Cross present antigen to CTL
Ligand Receptor: Ubiquitous preformed acid-labile protein associated ligand(s)
Interaction: Necrotic cells
Antigen References:

Poulin LF, et al. 2012. Blood 119:6052.