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Safety Data Sheet (SDS) PE Anti-mouse CD43 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD43 Antibody
1316025 50 µg $100.00       
1316030 200 µg $260.00       
Clone: S11
Isotype: Rat IgG2b
Reactivity: Mouse
Immunogen: Mouse plasmacytoma cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: Western blotting3. The S11 antibody reacts with pan-CD43.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Gaspari AA, et al. 1993. J. Invest. Dermatol. 100:247. (FC)
2. Merzaban JS, et al. 2005. J. Immunol. 174:4051. (FC)
3. Baecher-Allan CM, et al. 1993. Immunogenetics. 37:183. (WB)

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with either anti-mouse CD43 (clone S11) PE (filled histogram) or rat IgG2b PE isotype control (dashed histogram).



Description:

CD43, also known as Leukosialin and Ly48, is a 125 kD sialoprotein (glycosylated protein) expressed from 1.2 kBase mRNA in bone marrow-derived cells. This occurs early in development. Cells expressing CD43 include γ/δ T cells, macrophages, mature B cells, and dendritic cells. CD43 functions as an anti-adhesive surface molecule, promoted by antibody cross-linking, that releases the trailing edge of the cell during locomotion to allow movement of the cell body towards the lamellipodia. The intracellular distribution of CD43 is determined by binding to moesin, an intracellular membrane protein, which is in-turn bound in some manner to the actin cytoskeleton. Defects with CD43 function and expression retard cellular locomotion, resulting in a wide range of immune disorders. Wiscott-Alderich syndrome, and the varying degrees of its severity, is related to the dysregulation of CD43 expression.

Other Names: Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, gp115
Structure: Single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites; 115 kD and 130 kD glycoforms with different glycosylation and sialylation.
Distribution: Gamma/delta T cells, macrophages, mature B cells, and dendritic cells.
Function: Plays dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors.
Ligand Receptor: Moesin, CD54, E-selectin, Siglec-1.
Interaction: CD43 functions as an anti-adhesive surface molecule, promoted by antibody cross-linking, that releases the trailing edge of the cell during locomotion to allow movement of the cell body towards the lamellipodia.
Antigen References:

1. Van den Berg TK, et al. 2001. J. Immunol. 166:3637.
2. Moore T, et al. 1994. J. Immunol. 153:4978.
3. Onami TM, et al. 2002. J. Immunol. 168:6022.
4. Tong J, et al. 2004. J. Exp. Med. 199:1277.
5. Jones AT, et al. 1994. J. Immunol. 153:3426.
6. Matsumoto M, et al. 2005. J. Immunol. 175:8042.