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Safety Data Sheet (SDS) PE Anti-mouse PD-1H VISTA Antibody     Product Data Sheet (PDF)    
PE Anti-mouse PD-1H (VISTA) Antibody
1318535 25 µg $120.00       
1318540 100 µg $315.00       
Clone: MH5A
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: PD-1H- IgG Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: inhibition of graft vs host disease (GVHD), Western blotting, and immunohistochemical staining of paraffin embedded tissue sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Flies DB, et al. 2011. J. Immunol. 187:1537.

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with CD8 APC and PD-1H (clone MH5A) PE (top) or Armenian Hamster IgG PE isotype control (bottom).





Description:

PD-1H, also known as VISTA, is a 309 aa type I transmembrane protein, composed of seven exons. PD-1H has one Ig-V like domain, and its sequence is similar to the Ig-V domains of the members of CD28 and B7 families. PD-1H is expressed by a subset of T cells, macrophages, dendritic cells, neutrophils, and NK cells. It has been proposed that PD-1H can be useful to modulate the host immune response to allogeneic transplants. 

Other Names: PD-1 homolog, Dies1
Structure: Type I transmembrane protein, member of the CD28 family
Distribution: Macrophages, dendritic cells, neutrophils, NK cells, naïve and activated T cells
Function: Negative regulator of the immune response
Antigen References:

1. Flies DB, et al. 2011. J. Immunol. 187:1537.
2. Wang Li, et al. 2011. J. Exp Med. 208:577.