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Safety Data Sheet (SDS) Purified Anti-mouse Ki-67 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse Ki-67 Antibody
3862005 25 µg $70.00       
3862010 100 µg $170.00       
Clone: 16A8
Isotype: Rat IgG2a, κ
Reactivity: Mouse, Human
Immunogen: E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa.
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.

WB, ICFC - Quality tested
IF - Reported in the literature

Application Notes:

Ki-67 Staining Protocol:

1. Prepare 70% ethanol and chill at -20°C.
2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xg for 5 minutes.
3. Discard supernatant and loosen the cell pellet by vortexing.
4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.
5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.
6. Wash 3X with Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 106/ml.
7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.
8. Wash 2X with Cell Staining and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.

Recommended Usage:

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µl per 5 ml antibody dilution buffer for each mini-gel (0.5 µg/ml). Additionally, each lot of this antibody is quality control tested by our Ki-67 staining protocol below. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Medina-Reyes EI, et al. 2015. Environ Res. 136:424. PubMed
2. Guillaumond F, et al. 2015. PNAS. 112:2473. PubMed
3. Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed
4. Rodero MP, et al. 2014. J. Invest. Dermatol. 7:1991-7. PubMed

Total cell lysates (15 µg

Total cell lysates (15 µg protein) from Raw264.7, HeLa, and Daudi were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with mouse Ki-67 Antibody (clone 16A8) (upper). Proteins were visualized using a goat anti-rat IgG secondary antibody conjugated to HRP and chemiluminescence detection. β-actin Antibody (lower) was used as a loading control.


The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase, while in G0 phase the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.

Other Names: KIA, proliferation-related Ki-67 antigen
Structure: 325 kD protein containing a forkhead-associated domain (FHA) and 13 tandem repeats
Distribution: Nucleus and chromosome
Function: Required for cell cycle progression and proliferation
Interaction: Interacts with KIF15; binds to MKI67IP through FHA domain
Antigen References:

1. Starborg M, et al. 1996. J. Cell. Sci. 109:143.
2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.