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Safety Data Sheet (SDS) Purified Anti-His Tag Antibody     Product Data Sheet (PDF)    
Purified Anti-His Tag Antibody
3862505 25 µg $70.00       
3862510 100 µg $155.00       
3862525 1 mg $570.00       
Clone: J099B12
Isotype: Mouse IgG1, κ
Reactivity: All species (6x His tag epitope)
Immunogen: KLH-conjugated His-tag peptide
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, 0.09% NaN3.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application:

WB - Quality tested
IF, ICFC - Validated

Recommended Usage:

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.1-0.2 µg/ml antibody dilution buffer for each mini-gel. Immunofluorescence microscopy, suggested working dilution(s): Use 0.5-1.0 µg/ml antibody dilution buffer. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
2. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.

Insect cell supernatant expressing His-tagged

Insect cell supernatant expressing His-tagged mouse CCL22 was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-His Tag antibody (clone J099B12). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection.

293E cells transfected with His-tagged

293E cells transfected with His-tagged human pro-IL1β were probed with anti-His Tag antibody (clone J099B12). The cells were then detected by DyLight™ 594 conjugated anti-mouse IgG1 secondary (cytosol, red) and DAPI (nuclei, blue).



Description:

This antibody was raised against linear 8XHis tag. It reacts with His tag protein, either C, N, or internal His6 and up repeats. It can be used for Western blotting to detect linear His repeat (His6 or more). It can also be used for ICFC and IF staining of His-Tagged protein.

Other Names: Histidine Tag
Function: Recombinant poly-histidine tag detection