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Safety Data Sheet (SDS) PE Anti-mouse PIR-A/B Antibody     Product Data Sheet (PDF)    
PE Anti-mouse PIR-A/B Antibody
1320515 25 µg $90.00       
1320520 100 µg $240.00       
Clone: 6C1
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: Recombinant extracellular domains 1 and 2 of PIR-A
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications for the relevant formats include: Western Blotting1 and immunohistochemical staining of frozen tissue sections3.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Kubagawa H, et al. 1999. J. Exp. Med. 189:309. (WB)
2. Nakamura A, et al. 2004. Nat. Immunol. 5:623. (FC)
3. Masuda K, et al. 2005. EMBO J. 24:4052. (IHC)

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with CD19 Brilliant Violet 421™ and PIR-A/B (clone 6C1) PE (top) or rat IgG1, κ PE isotype control (bottom).





Description:

Paired-immunoglobulin-like receptor (PIR) is a type I transmembrane protein, containing six Ig-like domains. Two isoforms (PIR-A and PIR-B) have been reported for this molecule. PIR-A/B is expressed on B cells, mast cells, macrophages, granulocytes, eosinophils, and dendritic cells. PIR-A requires association with FcRγc for its cell surface expression and to act as an activating receptor. The ligand for PIR-A is MHC class I. PIR-B is an inhibitory receptor with 3 ITIM motifs on its cytoplasmic tail; when phosphorylated, they recruit the tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. MHC class I, Nogo, MAG, and OMgp are the ligands for PIR-B.

Other Names: PIRA1, PIR-B Leukocyte immunoglobulin-like receptor 3, LIR-3, GP91
Structure: Transmembrane proteins, six Ig-like domains. PIR-A associates with FcRγc, doesn't have functional motifs on its cytoplasmic domain. PIR-B contains 3 ITIM motifs on its cytoplasmic domain.
Distribution: B cells, macrophages, dendritic cells, mast cells, eosinophils.
Ligand Receptor: MHC class I, Nogo, MAG, OMgp.
Interaction: PIR-A: FcRγc
PIR-B: Lyn, PTPN6/SHP-1, PTPN11/SHP-2
Antigen References:

1. Matsushita H, et al. 2011. J. Biol. Chem. 286:25739.
2. Arita K, et al. 2011. J. Immunol. 186:7050.
3. Takai T, et al. 2011. J. Biomed. Biotechnol. 2011:275302.
4. Nakamura A, et al. 2004. Nat. Immunol. 5:623.