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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-mouse CD115 CSF-1R Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-mouse CD115 (CSF-1R) Antibody
1277555 25 µg $115.00       
1277560 100 µg $220.00       
Clone: AFS98
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: blocking of ligand binding1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Application References:

1. Sudo T, et al. 1995. Oncogene 11:2469.
2. Murayama T, et al. 1999. Circulation 99:1740.
3. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. PubMed

Thioglycolate-elicited C57BL/6 mouse peritoneal macrophages

Thioglycolate-elicited C57BL/6 mouse peritoneal macrophages stained with CD115 (clone AFS98) Alexa Fluor® 488 (filled histogram) or rat IgG2a, κ Alexa Fluor® 488 control (open histogram).



Description:

CSF-1R, also known as CD115 and M-CSFR, is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. This c-fms (Fms proto-oncogene) gene product's natural ligands include M-CSF and IL-34. Structural studies of CD115 have described an Ig-like extracellular domain, a transmembrane domain, an intracellular juxtamembrane domain, a split tyrosine kinase domain, and a C-terminal tail receptor. Receptor activation induces homodimerization in addition to phosphorylation and ubiquitination of intracellular residues. CD115 directly influences tissue macrophage and osteoclast differentiation and proliferation. It is expressed on monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, and osteoclasts.

Other Names: CSF-1R, M-CSFR, c-fms
Structure: Ig superfamily, 145 kD
Distribution: Monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, osteoclasts
Function: Growth factor receptor, tyrosine kinase
Ligand Receptor: Macrophage colony stimulating factor (M-CSF), IL-34
Antigen References:

1. Sudo T, et al. 1995 Oncogene 11:2469.
2. Murayama T, et al. 1999 Circulation 99:1740.
3. Goswami S, et al. 2005 Cancer Res. 65:5278.
4. Yu W, et al. 2008 J. Leuko. Biol. 84:852.


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