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Safety Data Sheet (SDS) APC Anti-mouse CD201 EPCR Antibody     Product Data Sheet (PDF)    
APC Anti-mouse CD201 (EPCR) Antibody
1307525 25 µg $115.00       
1307530 100 µg $290.00       
Clone: RCR-16
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: in vivo and in vitro blocking of protein C binding1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Centelles MN, et al. 2010. Thromb Haemost. 103:1239. (Block)

Mouse endothelial cell line, bEND.3,

Mouse endothelial cell line, bEND.3, was stained with mouse CD201 (clone RCR-16) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).



Description:

Endothelial protein C receptor (EPCR), also known as CD201, is a 46 kD N-glycosylated type I transmembrane protein, primarily expressed on the endothelial cells of arteries and veins in heart and lung. It is also expressed at high levels on a subset of hematopoietic stem cells and a subset of dendritic cells. CD201 functions as a primary receptor for protein C activation, and results in inhibition of both intrinsic and extrinsic coagulation pathways. It also plays an important role in many pathophysiologic processes, such as inflammation responses to infection, trauma, hematopoiesis, and autoimmune response. Deletion of the CD201 gene in knock-out mice leads to embryonic lethality before embryonic day 10, indicating that CD201 expression is critical for embryo development. In humans, mutations of CD201 have been associated with venous thromboembolism and myocardial infarction as well as with late fetal loss during pregnancy.

Other Names: Endothelial protein C receptor, protein C receptor, PROCR, activated protein C receptor, APC receptor
Structure: Type I transmembrane glycoprotein, 46 kD
Distribution: Endothelial cells, subset of hematopoietic stem cells, subset of dendritic cells
Function: Inhibits blood coagulation, apoptosis, inflammation, endothelial activation
Ligand Receptor: Protein C, activated protein C
Interaction: Protease-activated receptor 1 (Par-1)
Antigen References:

1. Kerschen E, et al. 2010. J. Clin. Invest. 120:3167.
2. Iwasaki H, et al. 2010. Blood 116:544.
3. Balazs AB, et al. 2006. Blood 107:2317.