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Safety Data Sheet (SDS) APC Anti-mouse CD73 Antibody     Product Data Sheet (PDF)    
APC Anti-mouse CD73 Antibody
1236045 25 µg $90.00       
1236050 100 µg $190.00       
Clone: TY/11.8
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: CHO cells transfected with mouse CD73
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981.
2. Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-paraffin)

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with CD3 APC/Cy7 and CD73 (clone TY/11.8) APC (top) or rat IgG1, κ APC isotype control (bottom).





Description:

CD73 (ecto-5`-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b+ myeloid cells. In spleen, it is largely expressed on T cells.

Other Names: Ecto-5'-nucleotidase
Structure: 69 kD GPI-anchored surface protein
Distribution: Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73
Function: A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates
Ligand Receptor: Nucleoside 5' monophosphate
Regulation: Expression is regulated by the development and maturation of leukocytes
Antigen References:

1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981.
2. Resta R, et al. 1998. Immunol. Rev. 161:95.