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Safety Data Sheet (SDS) FITC Anti-mouse/rat CD61 Antibody     Product Data Sheet (PDF)    
FITC Anti-mouse/rat CD61 Antibody
1121525 50 µg $85.00       
1121530 500 µg $255.00       
Clone: 2C9.G2 (HMß3-1)
Isotype: Armenian Hamster IgG
Reactivity: Mouse, Rat
Immunogen: Vitronectin receptor protein from the mouse T-cell hybridoma 2B4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: blocking of ligand binding1-4, activation of αv β3 integrin signaling5, and immunohistochemical staining of acetone-fixed frozen sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Kieffer N, et al. 1990. Annu. Rev. Cell Biol. 6:329. (Block)
2. Piali L, et al. 1995. J. Cell Biol. 130:451. (Block)
3. Ashkar S, et al. 2000. Science 287:860. (Block)
4. Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block)
5. Moulder K, et al. 1991. J. Exp. Med. 173:343. (Activ)
6. Carlson TR, et al. 2008.135:2193. PubMed
7. Yamaji D, et al. 2009. Genes Dev. 23:2382. PubMed
8. Olover HK, et al. 2014. Mol Pharmacol. 85:921. PubMed
9. Roarty K, et al. 2015. J Cell Sci. 208:351. PubMed

C57BL/6 mouse bone marrow cells

C57BL/6 mouse bone marrow cells stained with HMβ3-1 FITC



Description:

CD61 is a 110 kD integrin β chain also known as β3 integrin or gpIIIa. It associates with the integrin αv chain (CD51) to form the vitronectin receptor. In addition, CD61 can associate with the integrin αIIb chain (CD41) to form the gpIIb/IIIa complex. CD61 is expressed on platelets, megakaryocytes, endothelium, smooth muscle, a subset of B cells, myeloid cells, osteoclasts, and mast cells. CD61, in conjunction with CD41 or CD51, mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, and von Willebrand factor. Leukocyte-endothelial adhesion is mediated by the binding of αv3 integrin or vitronectin receptor to CD31 (PECAM-1).

Other Names: β3 integrin, Integrin β3 chain, gpIIIa
Structure: Integrin family, 110 kD
Distribution: Platelets, megakaryocytes, endothelium, smooth muscle, activated T cells, subset of B cells, monocytes, macrophages, osteoclasts, mast cells
Function: Attachment to matrix
Ligand Receptor: Fibrinogen, von Willebrand factor, fibronectin
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Phillips DR, et al. 1991. Cell 65:359.
3. Felding-Habermann B, et al. 1993. Curr. Opinion Cell Biol. 5:864.