Sony Biotechnology Inc. Sony Biotechnology Inc.cart
About Us Products What's New Library News Calendar Contact Us Place Order
Safety Data Sheet (SDS) PerCP/Cy55 Anti-mouse PD-1H VISTA Antibody     Product Data Sheet (PDF)    
PerCP/Cy5.5 Anti-mouse PD-1H (VISTA) Antibody
1318555 25 µg $155.00       
1318560 100 µg $320.00       
Clone: MH5A
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: PD-1H- IgG Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: inhibition of graft vs host disease (GVHD), Western blotting, and immunohistochemical staining of paraffin embedded tissue sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application References:

1. Flies DB, et al. 2011. J. Immunol. 187:1537.

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with CD8 FITC and PD-1H (clone MH5A) PerCP/Cy5.5 (top) or Armenian hamster IgG PerCP/Cy5.5 isotype control (bottom).





Description:

PD-1H, also known as VISTA, is a 309 aa type I transmembrane protein, composed of seven exons. PD-1H has one Ig-V like domain, and its sequence is similar to the Ig-V domains of the members of CD28 and B7 families. PD-1H is expressed by a subset of T cells, macrophages, dendritic cells, neutrophils, and NK cells. It has been proposed that PD-1H can be useful to modulate the host immune response to allogeneic transplants. 

Other Names: PD-1 homolog, Dies1
Structure: Type I transmembrane protein, member of the CD28 family
Distribution: Macrophages, dendritic cells, neutrophils, NK cells, naïve and activated T cells
Function: Negative regulator of the immune response
Antigen References:

1. Flies DB, et al. 2011. J. Immunol. 187:1537.
2. Wang Li, et al. 2011. J. Exp Med. 208:577.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
COMMERCIAL USE shall include:

  • sale, lease, license or other transfer of the material or any material derived or produced from it;
  • sale, lease, license or other grant of rights to use this Material or any material derived or produced from it;
  • use of this material to perform services for a fee for third parties.

IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE RETURN THIS MATERIAL, UNOPENED TO Sony Biotechnology Inc., 2100 South Oak Street, Champaign, IL 61820, USA AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED.