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Safety Data Sheet (SDS) Purified Anti-mouse CD369 Dectin-1/CLEC7A Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD369 (Dectin-1/CLEC7A) Antibody
1321505 25 µg $70.00       
1321510 100 µg $180.00       
Clone: RH1
Isotype: Rat IgG1, κ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Mouse Dectin-1-transfected cells were stained

Mouse Dectin-1-transfected cells were stained with purified anti-mouse CD369 (Dectin-1/CLEC7A, clone RH1) (filled histogram) or rat IgG1, κ isotype control (open histogram), followed by anti-rat IgG FITC.



Description:

CD369 (Dectin-1), also known as CLEC7A, is a 43 kD type II transmembrane protein.  The extracellular portion of Dectin-1 contains one C-type lectin domain, and its cytoplasmic tail has one immunoreceptor tyrosine-based activation motif (ITAM). Dectin-1 binds β-1,3-linked and β-1,6-glucans (abundant in the cell wall of fungi and yeast), mediates phagocytosis, and induces cytokine production and oxidative burst. It is also involved in TLR2-mediated NF-kB activation. Dectin-1 is expressed by macrophages, neutrophils, dendritic, and Langerhans cells.

Other Names: BGR, βGR, CD369
Structure: 43 kD, type II transmembrane protein, 1 C-type lectin domain, 1 ITAM motif
Distribution: Macrophages, dendritic cells, Langerhans cells
Function: Phagocytosis, induces cytokine production and oxidative burst
Ligand Receptor: β-1,3-linked and β-1,6-linked glucans
Antigen References:

1. Iliev ID, et al. 2012. Science 336:1314.
2. Saijo S and Iwakura Y. 2011. Int. Immunol. 23:467.
3. Reid DM, et al. 2009. Curr. Opin. Immunol. 21:30.
4. Saijo S, et al. 2007. Nat. Immunol. 8:39.