Sony Biotechnology Inc. Sony Biotechnology Inc.cart
About Us Products What's New Library News Calendar Contact Us Place Order
Safety Data Sheet (SDS) Purified Anti-human/mouse/rat PCNA Antibody     Product Data Sheet (PDF)    
Purified Anti-human/mouse/rat PCNA Antibody
2139505 25 µg $55.00       
2139510 100 µg $100.00       
Clone: PC10
Isotype: Mouse IgG2a, κ
Reactivity: Human, Mouse, Rat, Cross-Reactivity: Other species
Immunogen: Recombinant rat PCNA
Formulation: Phosphate-buffered solution, pH7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.

ICFC - Quality tested
IF, IHC - Validated
IP, WB - Reported in the literature

Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining2,5,6 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections, immunoprecipitation, immunofluorescence microscopy9, and Western blotting10.
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Ogata K, et al. 1985. J. Immunol. 135:2623.
2. Garcia R, et al. 1989. Am. J. Pathol. 134:733.
3. Landberg G, et al. 1990. Exp. Cell. Res. 187:111.
4. Waseem N, et al. 1990. J. Cell Sci. 96:121.
5. Yu C, et al. 1991. Histopathology 19:29.
6. Wilkins B, et al. 1992. J. Pathol. 166:45.
7. Yang W, et al. 1996. Human Pathol. 27:70.
8. Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329.
9. Chou HYE, et al. 2006. J. Biol. Chem. 10:1074.
10. Fulvio MD, et al. 2006. Oncogene 25:3932.
11. Eswarakumar VP and Schlessinger J. 2007. Proc. Natl. Acad. Sci. USA 104:3937.
12. Henkels KM, et al. 2009. Biochem Biophys Res Commun. 389:224. PubMed
13. Brobeli A, et al. 2010. Blood Cells Mol Dis. 45:159. PubMed
14. Wallace HA, et al. 2014. Development. 141:1332. PubMed
15. Mizokami A, et al. 2014. Bone. 69:68. PubMed

Hela cell nuclear extract was

Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

HeLa cells were fixed with

HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml of purified anti-human/mouse/rat PCNA (clone PC10) in blocking buffer overnight at 4°C, followed by DyLight™ 594 anti-mouse IgG (red) and Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with 20X objective.


The PC10 monoclonal antibody reacts with proliferating cell nuclear antigen also known as PCNA or the DNA polymerase δ auxiliary protein. PCNA is a 36 kD trimeric ring that acts as a DNA-polymerase sliding clamp expressed in the nucleus of all proliferating cells. A prime function of PCNA appears to be increasing DNA polymerase δ processibility during elongation of the leading strand. PCNA is a useful marker for DNA synthesis and is highly conserved among most species, thus highlighting the very broad reactivity of this antibody.

Other Names: Proliferating Cell Nuclear Antigen, DNA Polymerase δ Auxiliary Protein
Structure: DNA-polymerase sliding clamp, trimeric ring; 36 kD
Distribution: Nuclear, all proliferating cells
Function: RAD6-dependent DNA repair pathway; increases DNA polymerase δ processibility during elongation of the leading strand
Ligand Receptor: Ubiquitination, Sumoylation
Interaction: PCNA, DNA polymerase δ, Rad6, Rad18, UBC9, MMS2, UBC13, RAD5
Antigen References:

1. Travali S, et al. 1989. J. Biol. Chem. 264:7466.
2. Waseem N, et al. 1990. J. Cell Sci. 96:121.
3. Hall P, et al. 1990. J. Pathol. 162:285.
4. Landberg G, et al. 1991. Cancer Res. 51:4570.
5. Woods A, et al. 1991. Histopathol. 19:21.
6. Hoege C, et al. 2002. Nature 419:135.
7. Yue H, et al. 2003. World J. Gastroenterol. 9:377.
8. Shan B, et al. 2003. J. Biol. Chem. 278:44009.