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Safety Data Sheet (SDS) Brilliant Violet 510 Anti-mouse Ly-6A/E Sca-1 Antibody     Product Data Sheet (PDF)    
Brilliant Violet 510™ Anti-mouse Ly-6A/E (Sca-1) Antibody
1140645 125 µl $170.00       
Clone: D7
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: IL-2-dependent mouse T-cell line (CTL-L)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 510™ and unconjugated antibody.
Concentration: Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed, paraffin-embedded samples9.

The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14 

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Application References:

1. Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP)
2. Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB)
3. Codias EK, et al. 1990. J. Immunol. 144:2197.
4. Malek TR, et al. 1986. J. Exp. Med. 164:709.
5. Codias EK, et al. 1990. J. Immunol. 145:1407.
6. Ivanov V, et al. 1994. J. Immunol. 153:2394.
7. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
8. Fleming T, et al. 1994. J. Immunol. 153:1955.
9. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC)
10. Umland O, et al. 2007. J. Immunol. 178:4147.
11. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed
12. Pronk CJ, et al. 2011. J. Exp Med. PubMed
13. English A, et al. 2000. J. Immunol. 165:3763. (IHC)
14. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294. 

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with Ly-6A/E (clone D7) Brilliant Violet 510™ (filled histogram) or rat IgG2a, κ Brilliant Violet 510™ isotype control (open histogram).

C57BL/6 mouse bone marrow cells

C57BL/6 mouse bone marrow cells were stained with Ly-6A/E (clone D7) Brilliant Violet 510™ (filled histogram) or rat IgG2a, κ Brilliant Violet 510™ isotype control (open histogram). Data shown was gated on lymphoid cell population.



Description:

Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.

Other Names: Sca-1
Structure: Ly-6 multigene family, 18 kD
Distribution: Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells
Function: Regulates B and T cell responses
Antigen References:

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.