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Safety Data Sheet (SDS) APC Anti-mouse CD154 Antibody     Product Data Sheet (PDF)    
APC Anti-mouse CD154 Antibody
1132545 25 µg $75.00       
1132550 100 µg $215.00       
Clone: MR1
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Activated mouse Th1 clone D1.6
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining1,2 of acetone-fixed frozen sections, and in vitro and in vivo blocking of ligand binding3-5. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody or biotinylated anti-Armenian hamster IgG second step, followed by SAv-PE ).

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Lettesjö H, et al. 2000. J. Immunol. 165:4095. (IHC)
2. Dunn RJ, et al. 1997. J. Histochem. Cytochem. 45:129. (IHC)
3. Noelle RJ, et al. 1992. P. Natl. Acad. Sci. USA 89:6550. (Block)
4. Roy M, et al. 1995. Eur. J. Immunol. 25:596. (Block)
5. Foy TM, et al. 1994. J. Exp. Med. 180:157. (Block)
6. Lawson BR, et al. 2007. J. Immunol. 178:5366.
7. Inoue M, et al. 2014. PNAS. 111:5295. PubMed

Enriched C57BL/6 mouse splenic T

Enriched C57BL/6 mouse splenic T cells were stimulated with PMA + ionomycin for 6 hours and then stained with CD154 (clone MR1) APC (filled histogram) or Armenian hamster IgG APC isotype control (open histogram).



Description:

CD154 is a 39 kD TNF superfamily member also known as CD40 ligand, gp39, T-BAM, TRAP, and Ly-62. CD154 is an accessory molecule expressed predominantly on activated CD4+ lymphocytes that bind CD40. CD154 plays an important role in T-B cell costimulation. The MR1 antibody has been reported to inhibit the activation of T and B lymphocytes in vitro and antigen-specific lymphocyte responses in vivo.

Other Names: CD40 ligand (CD40L), gp39, T-BAM, TRAP, Ly-62, TNFSF5
Structure: TNF superfamily, 39 kD
Distribution: Activated CD4+ T cells
Function: T-B cell costimulation
Ligand Receptor: CD40
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Noelle RJ, et al. 1992. P. Natl. Acad. Sci. USA 89:6550.
3. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
4. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.