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Safety Data Sheet (SDS) Pacific Blue Anti-mouse/rat/human CD27 Antibody     Product Data Sheet (PDF)    
Pacific Blue™ Anti-mouse/rat/human CD27 Antibody
1221085 25 µg $90.00       
1221090 100 µg $270.00       
Clone: LG.3A10
Isotype: Armenian Hamster IgG
Reactivity: Mouse, Rat, Human
Immunogen: Armenian hamster fibroblast line ARHO12 transfected with mouse CD27 cDNA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: functional assay1,3 and immunohistomecial staining of acetone-fixed frozen sections2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Application References:

1. Gravestein LA, et al. 1995. Int. Immunol. 7:551. (FA)
2. Gravestein LA, et al. 1996. J. Exp. Med. 184:675. (IHC)
3. Takeda K, et al. 2000. J. Immunol. 164:1741. (FA)
4. Welner RS, et al. 2009. J. Immunol. 183:7768. PubMed
5. Vicetti Miguel RC, et al. 2012. J. Immunol. 189:3449. PubMed
6. White CA, et al. 2014. J Immunol. 193:5933. PubMed

C57Bl/6 mouse splenocytes were stained

C57Bl/6 mouse splenocytes were stained with CD27 (clone LG.3A10) Pacific Blue™ (filled histogram) or Armenian Hamster IgG Pacific Blue™ isotype control (open histogram).



Description:

CD27 is also known as S152 and T14. A member of the tumor necrosis factor receptor (TNFR) superfamily, it is a 45 kD protein expressed on peripheral T cells, memory B cells, NK cells, and thymocyte subset. Through its ligand, CD70, CD27 plays a key role in T cell and B cell interactions. Additionally, ligation of CD27 on naïve T cells may be important in their maturation to effector cells.

Other Names: T14, S152, Tp55, TNFRSF7
Structure: TNFR superfamily, 45 kD
Distribution: Peripheral blood T cells, memory B cells, NK cells, thymocyte subset
Function: Costimulatory signal for T and B cell activation, T cell development
Ligand Receptor: CD70
Interaction: TRAF2, TRAF5, CD27-binding protein (Siva)
Antigen References:

1. Hintzen RQ, et al. 1994. Immunol. Today 15:307.
2. Akiba H, et al. 1998. J. Biol. Chem. 273:13353.
3. Kobata T, et al. 1995. P. Natl. Acad. Sci. USA 92:11249.
4. Hendriks J, et al. 2000. Nat. Immunol. 1:433
5. Hintzen RQ, et al. 1994. Int. Immunol. 6:477
6. Camerini D, et al. 1991. J. Immunol. 147:3165


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.