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Safety Data Sheet (SDS) PerCP/Cy55 Anti-mouse CD138 Syndecan-1 Antibody     Product Data Sheet (PDF)    
PerCP/Cy5.5 Anti-mouse CD138 (Syndecan-1) Antibody
1312545 25 µg $155.00       
1312550 100 µg $320.00       
Clone: 281-2
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse mammary gland epithelial cell line NMuMG
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3 and formalin-fixed paraffin embedded tissue4 and immunofluorescent staining2,3.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application References:

1. Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC)
2. Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF)
3. Li Q, et al. 2002. Cell 111:635. (IF, IHC)
4. McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)

C57BL/6 mouse bone marrow cells

C57BL/6 mouse bone marrow cells were stained with CD19 FITC and CD138 (clone 281-2) PerCP/Cy5.5 (top) or rat IgG2a, κ PerCP/CY5.5 isotype control (bottom). Data shown is gated on lymphoid cell population.





Description:

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant plasma cells, pre-B cells, mesenchymal cells, epithelial cells, and endothelial cells.

Other Names: Syndecan-1
Structure: Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD
Function: Cell adhesion, cell motility, cell migration, cell proliferation
Antigen References:

1. Zong F, et al. 2011. PLoS ONE 6:e14816.
2. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
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