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Safety Data Sheet (SDS) APC Anti-mouse CD366 Tim-3 Antibody     Product Data Sheet (PDF)    
APC Anti-mouse CD366 (Tim-3) Antibody
1198525 25 µg $80.00       
1198530 100 µg $220.00       
Clone: RMT3-23
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for relevant formats) include: in vitro1 and in vivo2 blocking of Tim-3, and immunohistochemical staining of frozen sections2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Nakae S, et al. 2007. Blood 110(7):2565-8. (FC, Block)
2. Oikawa T, et al. 2006. J. Immunol. 177(7):4281-7. (FC, Block, IHC)

Mouse Tim-3 transfected cells were

Mouse Tim-3 transfected cells were stained with anti-mouse CD366 (Tim-3, clone RMT3-23) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).



Description:

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b+ macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Other Names: T cell immunoglobulin and mucin domain containing 3 protein, hepatitis virus cellular receptor 2, CD366
Structure: Transmembrane protein containing immunoglobulin domain and mucin-like domain; predicted molecular weight 31 kD; can exist as a soluble form lacking mucin and transmembrane domains
Distribution: Expressed on activated Th1 lymphocytes and CD11b+ macrophages
Function: May play a role in the development of immune responses and the development of Th1-mediated responses
Ligand Receptor: Putative ligand on resting CD4+ lymphocytes
Antigen References:

1. Sabatos CA, et al. 2003. Nat. Immunol. 4:1102.
2. Sanchez-Fueyo A, et al. 2003. Nat. Immunol. 4:1102.
3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
4. Mooney L, et al. 2002. Nature 415:536.