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Safety Data Sheet (SDS) Purified Anti-mouse Isthmin-1 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse Isthmin-1 Antibody
1325005 25 µg $120.00       
1325010 100 µg $270.00       
Clone: B054B6
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Full length mouse Isthmin 1 recombinant protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

ICFC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Mouse Isthmin-1 transfected HEK-293E cells

Mouse Isthmin-1 transfected HEK-293E cells were fixed, permeabilized, and then stained with purified Isthmin-1 (clone B054B6, filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG FITC.



Description:

Isthmin is a secreted protein, and it was initially identified in the Xenopus midbrain-hindbrain organizer (MHB) or isthmus organizer, where it is highly expressed. The MHB is an important signaling center in vertebrates and regulates the polarized morphological differentiation of the adjacent tectum and cerebellum. Mouse Isthmin is a 454 amino acid protein containing a Thrombospondin Type 1 Repeat (TSR) domain in the central region and an Adhesion-associated domain in MUC4 and Other Proteins (AMOP) domain at the C-terminal. The TSR domains are highly conserved with 98% identity between mouse and human; 87-88% identity between mouse and Xenopus. The C-terminal AMOP domains are also highly conserved with 99% identity between mouse and human; 91% identity between mouse and Xenopus. Mouse isthmin nucleotide sequence is similar to the human chromosome 20 open reading frame 82 (C20orf82). The Ism1 gene is conserved in human, chimpanzee, dog, cow, chicken, and zebrafish. Isthmin has angiostatic activity in vitro and in vivo. Isthmin inhibits EC capillary network formation mainly by interfering with the early stages of in vitro angiogenesis on matrigel. Isthmin inhibits VEGF-induced EC proliferation and induces apoptosis in the presence of VEGF. Overexpression of isthmin in B16 melanoma inhibits tumor growth and tumor angiogenesis in mice. Knockdown of isthmin in zebrafish embryos leads to abnormal intersegmental vessel (ISV) formation in the trunk.

Other Names: Ism1, BATAM
Structure: 454 aa with a predicted molecular weight of 51 kD.
Distribution: Isthmin is highly expressed in the midbrain-hindbrain organizer.
Interaction: Isthmin interacts with endothelial cells.
Antigen References:

1. Pera EM, et al. 2002. Mech. Dev. 116:169.
2. Xiang W, et al. 2009. J. Cell. Mol. Med. 15:359.
3. Zhang Y, et al. 2011. Cell Death. Dis. 2:e153.