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Safety Data Sheet (SDS) PE Anti-mouse DR3 TNFRSF25 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse DR3 (TNFRSF25) Antibody
1322025 25 µg $130.00       
1322030 100 µg $300.00       
Clone: 4C12
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Mtnfr25-Ig fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

4C12 is an agonistic antibody. It leads to rapid and selective proliferation of pre-existing Tregs in vivo through TCR engagement with MHC class II (MHC-II) and IL-2 signaling. We recommend our LEAF™ purified 4C12 antibody for agonist or in vivo studies.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Fang L, et al. 2008. J. Exp. Med. 205:1037.
2. Wolf D, et al. 2012. Transplantation. 94:569. PubMed
3. Schreiber TH, et al. 2012. J. Immunol. 189:3311.
4. Longman RS, et al. 2014. J Exp Med. 211:1571. PubMed

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with CD4 APC and DR3 (clone 4C12) PE (top) or Armenian hamster IgG PE isotype control (bottom).





Description:

DR3, also known as TNFRSF25, lymphocyte-associated receptor of death (LARD), WSL-1, TRAMP, TR3 and APO3, is a 55 kD glycoprotein belonging to the TNF receptor superfamily (TNFRSF). TNFSF25 is expressed at low level on CD4-positive, CD8-positive T cells and NKT cells. It has been shown to stimulate NF-κB activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domains containing adaptor proteins. Recently, it has been shown that TNFRSF25 is also expressed on Treg cells and regulates Treg proliferation.

Other Names: Apo-3, LARD, TNFR25, TRAMP, WSL-1, DR3
Structure: 55 kD glycoprotein, contains one death domain and four TNFR-Cys repeats
Distribution: CD4, CD8, NKT and Tregs at low level
Function: Plays a role in lymphocyte homeostasis, induces NFKB activation and apoptosis
Ligand Receptor: TL1-A
Antigen References:

1. Taraban VY, et al. 2011. J. Clin. Invest. 121:463.
2. Slebioda TJ, et al. 2011. Eur. J. Immunol. 41:2606.
3. Schreiber TH, et al. 2010. J. Clin. Invest. 120:3629.
4. Reddy PB, et al. 2012. J. Virol. 2012. [Epub].