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Safety Data Sheet (SDS) APC Anti-mouse CD134 OX-40 Antibody     Product Data Sheet (PDF)    
APC Anti-mouse CD134 (OX-40) Antibody
1197065 25 µg $100.00       
1197070 100 µg $260.00       
Clone: OX-86
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: Recombinant mouse OX-40-CD4 chimeric protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Clone OX-86 has been reported to act as an agonist and stimulate OX-40.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Higgins LM, et al. 1999. J. Immunol. 162:486. (FC, IHC)
2. Al-Shamkhani A, et al. 1996. Eur. J. Immunol. 26:1695. (Costim)
3. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed

Con A-stimulated (3 days) C57BL/6

Con A-stimulated (3 days) C57BL/6 splenocytes were stained with CD134 (clone OX-86) APC (filled histogram) or rat IgG1, κ APC isotype control (open histogram).



Description:

CD134 is a type I integral membrane protein also known as OX-40, ACT35, and tumor necrosis factor receptor superfamily member 4 (TNFRSF4). This receptor is expressed on activated CD4+ and CD8+ T cells and B cells. The OX-40 receptor binds to the OX-40 ligand (CD252) to provide a costimulatory signal that is independent of CD28. Blockade of OX40-OX40 ligand interactions has been shown to ameliorate experimental EAE and inflammatory bowel disease, which implies that these interactions are important in the pathogenesis of some autoimmune diseases.

Other Names: TNFRSF4, ACT35, OX-40
Structure: TNF receptor superfamily, 50 kD
Distribution: Activated CD4+ and CD8+ T cells, activated B cells
Function: Receptor for OX-40 ligand, provides co-stimulatory signal for lymphocyte proliferation independent of CD28; thought to play a role in the pathogenesis of some autoimmune diseases
Ligand Receptor: OX-40 ligand
Antigen References:

1. Al-Shamkhani A, et al. 1996. Eur. J. Immunol. 26:1695.
2. Weinberg AD, et al. 1999. J. Immunol. 162:1818.
3. Akira H, et al. 1999. J. Immunol. 162:7058.
4. Pippig SD, et al. 1999. J. Immunol. 163:6520.
5. Higgins LM, et al. 1999. J. Immunol. 162:486.