The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
Clone OX-86 has been reported to act as an agonist and stimulate OX-40.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Higgins LM, et al. 1999. J. Immunol. 162:486. (FC, IHC) 2. Al-Shamkhani A, et al. 1996. Eur. J. Immunol. 26:1695. (Costim) 3. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Con A-stimulated (3 days) C57BL/6 splenocytes were stained with CD134 (clone OX-86) APC (filled histogram) or rat IgG1, κ APC isotype control (open histogram).
CD134 is a type I integral membrane protein also known as OX-40, ACT35, and tumor necrosis factor receptor superfamily member 4 (TNFRSF4). This receptor is expressed on activated CD4+ and CD8+ T cells and B cells. The OX-40 receptor binds to the OX-40 ligand (CD252) to provide a costimulatory signal that is independent of CD28. Blockade of OX40-OX40 ligand interactions has been shown to ameliorate experimental EAE and inflammatory bowel disease, which implies that these interactions are important in the pathogenesis of some autoimmune diseases.
TNFRSF4, ACT35, OX-40
TNF receptor superfamily, 50 kD
Activated CD4+ and CD8+ T cells, activated B cells
Receptor for OX-40 ligand, provides co-stimulatory signal for lymphocyte proliferation independent of CD28; thought to play a role in the pathogenesis of some autoimmune diseases
1. Al-Shamkhani A, et al. 1996. Eur. J. Immunol. 26:1695. 2. Weinberg AD, et al. 1999. J. Immunol. 162:1818. 3. Akira H, et al. 1999. J. Immunol. 162:7058. 4. Pippig SD, et al. 1999. J. Immunol. 163:6520. 5. Higgins LM, et al. 1999. J. Immunol. 162:486.
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