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Safety Data Sheet (SDS) PE Anti-mouse CD124 IL-4Ralpha Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD124 (IL-4Rα) Antibody
1324015 25 µg $120.00       
1324020 100 µg $270.00       
Clone: I015F8
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: mIL4Ra Fc
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

C57BL/6 mouse splencotyes were stained

C57BL/6 mouse splencotyes were stained with B220 APC and CD124 (clone I015F8) PE (top) or rat IgG2b, κ PE isotype control (bottom).





Description:

CD124, a subunit of IL-4R, is a 140 kD transmembrane glycoprotein. It associates with either the common γ-chain (CD132) to form the type I IL-4R complex, which specifically binds IL-4, or with IL-13Ra1 to form the type II IL-4R heterodimeric complex, which binds and transduces signals from either IL-4 or IL-13.  A truncated form of IL-4Rα exists in soluble form in biological fluids. CD124 is expressed on mouse late precursors and mature B cells, T cells, monocytes, and has low expression on granulocytes.  In B cells, CD124 can bind IL-4 and IL-13 and regulate IgE antibody production. In T cells, the type I IL-4R (IL-4R/gC) is mostly responsible for Th2 cell expansion by mediating IL-4-dependent activation of the transcription factors in hematopoietic cells. The type II IL-4R (IL-4R/IL-13Ra1) is the main route for nonhematopoietic cell responses to IL-4 or IL-13.

Other Names: IL-4R, IL-4 receptor α subunit
Structure: 140 kD type I transmembrane glycoprotein, associates with common γ chain or IL-13 receptor alpha-1 subunit
Distribution: B cells, T cells, monocytes, and low levels on granulocytes.
Function: Regulates IgE antibody production in B cells, Th2 cell expansion, and IL-4 or IL-13 induced response in non-hematopoietic cells
Ligand Receptor: IL-4, IL-13
Interaction: STAT6
Antigen References:

1. Kashiwada M, et al. 2001. J. Immunol. 167:6382.
2. Gilmour J, et al. 2008. J. Immunol. 124:437.
3. Hage T, et al. 1999. Cell 97:271.