The antibody was purified by affinity chromatography and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
ELISA Detection1-3 or ELISPOT Detection4-6: The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody as capture antibody and recombinant mouse IL-2 as the standard. Flow Cytometry8-10: The fluorochrome-labeled JES6-5H4 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations. Neutralization1,7:
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Abrams J, et al. 1992. Immunol. Rev. 127:5. 2. Sander B, et al. 1993. J. Immunol. Meth. 166:201. 3. Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20. 4. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. 5. Mo X, et al. 1995. J. Virol. 69:1288. 6. Karulin A, et al. 2000. J. Immunol. 164:1862. 7. Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons New York. Unit 6.28. 8. Ko SY, et al. 2005. J. Immunol. 175:3309. PubMed 9. Kang SS and Allen PM. 2005. J. Immunol. 174:5382. 10. Lawson BR, et al. 2007. J. Immunol. 178:5366. 11. Cabrera-Perez J, et al. 2015. J Immunol. 194:1609. PubMed
PMA+ionomycin-stimulated C57BL/6 mouse splenocytes (in the presence of monensin for 6 hours), were stained with CD3 FITC, fixed, permeabilized, and then stained with IL-2 (clone JES6-5H4) PE/Cy7 (top) or rat IgG2b, κ PE/Cy7 isotype control (bottom).
IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.
Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
Cytokine; 15-30 kD (Mammalian)
Upregulated by NFAT; downregulated by TCF-8, CIF (colostrum inhibitory factor)
T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Taniguchi T, et al. 1993. Cell 73:5. 3. Nistico G. 1993. Prog. Neurobiol. 40:463. 4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.
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