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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-NFATc1 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-NFATc1 Antibody
3848015 25 µg $120.00       
3848020 100 µg $290.00       
Clone: 7A6
Isotype: Mouse IgG1
Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein of human NFATc1 amino acids 197-304.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested
IF - Reported in the literature

Application Notes:

7A6 antibody detects endogenous human NFATC1 in Western blot with an approximate molecular weight of 100 - 120 kD (different isoforms) . The optimal concentration should be determined by titration for each individual assay of interest.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Application References:

1. Timmerman LA, et al. 1997. J. Immunol. 159:2735. (IF)
2. Brandt C, et al. 2010. Cytometry A. 77:607. (FC)

Human peripheral blood lymphocytes were

Human peripheral blood lymphocytes were treated with pre-chilled 1% PFA plus 70% ethanol, then intracellularly stained with NFATc1 (clone 7A6) Alexa Fluor® 488 (filled histogram) or mouse IgG1, κ Alexa Fluor® 488 isotype control (open histogram).



Description:

The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. The protein complex consists of NFAT1, NFAT2 (NFATc1 or NFATc), NFAT3, and NFAT4. All members of this family are transcription factors with a Rel homology domain and regulate gene transcription in concert with AP-1 (Jun/Fos) to orchestrate an effective immune response. NFAT proteins are predominantly expressed in cells of the immune system but are also expressed in skeletal muscle, keratinocytes and adipocytes, regulating cell differentiation programs in these cells. In resting cells, NFAT proteins are heavily phosphorylated and localized in the cytoplasm. Increased intracellular calcium concentrations activate the calcium/calmodulin-dependent serine phosphatase calcineurin, which dephosphorylates NFAT proteins, resulting in their subsequent translocation to the nucleus.

Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Five transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes.

Other Names: Nuclear factor of activated T-cells, cytoplasmic 1, NFAT transcription complex cytosolic component, NFAT2, NFATc
Structure: 943 amino acids with predicted molecular weight of 101 kD.
Distribution: Cytoplasm, Nucleus. NFATc1 is cytoplasmic in the phosphorylated form and nuclear after calcineurin-mediated dephosphorylation controlled activation.
Antigen References:

1. Zhao Q, et al. 2010. Int. J. Biochem. Cell Biol. 42:576.
2. Hoey T, et al. 1995. Immunity 2:461.
3. Northrop JP, et al. 1993. J. Biol. Chem. 268:2917.
4. Hogan PG, et al. 2003. Genes Dev. 17:2205.
5. Shaw KT, et al. 1995. P. Natl. Acad. Sci. USA 92:11205.
6. Serfling E, et al. 2007. Sci. STKE 138:pe42.


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