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Safety Data Sheet (SDS) Purified Anti-mouse CD304 Neuropilin-1 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD304 (Neuropilin-1) Antibody
1326005 25 µg $100.00       
1326010 100 µg $220.00       
Clone: 3E12
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Extracellular region of mouse CD304
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
2. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
3. Verhagen J, et al. 2014. PLoS One. 9e:108023. (FC) PubMed

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with purified CD304 (clone 3E12, top) or purified rat IgG2a, κ isotype control (bottom), followed by anti-rat IgG PE. Splenocytes were then stained with mouse CD4 APC.





Description:

CD304, also known as neuropilin-1, is a 140 kD type I transmembrane protein. Its extracellular region contains two CUB, two FV/FVIII, and one MAM domain. It is expressed by natural regulatory T cells (nTreg), a subset of invariant natural killer T cells (iNKT), endothelial cells, and neurons. Neuropilin-1 stabilizes the interaction between Tregs and dendritic cells, contributes to the recruitment of tumor-infiltrating Tregs in response to tumor-derived VEGF, and influences the process of angiogenesis and axon guidance. The ligands of CD304 are VEGF165 and semaphorin-3A.

Other Names: NRP-1, VEGFR165 receptor
Structure: Type I transmembrane protein, 140 kD, 2 complement binding domains (CUB), 2 coagulation factor V/VIII homology domains (FV/FVIII), one meprin, A5, receptor tyrosine phosphatase domain (MAM)
Distribution: Natural regulatory T cells (nTreg), subset of invariant natural killer T cells (iNKT), endothelial cells, neurons
Function: Angiogenesis, axon guidance, modulation of the immune response
Ligand Receptor: VEGF165, semaphorin-3A
Antigen References:

1. Yadav M, et al. 2012. J. Exp. Med. 209:1713.
2. Weiss JM, et al. 2012. J. Exp. Med. 209:1723.
3. Hansen W, et al. 2012. J. Exp. Med. 209:2001.
4. Milpied P, et al. 2011. Blood 118:2993.