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Safety Data Sheet (SDS) PE Anti-mouse CD284 TLR4 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD284 (TLR4) Antibody
1327015 25 µg $105.00       
1327020 100 µg $210.00       
Clone: SA15-21
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: LPS-stimulated TLR4/MD-2/CD14 cotransfected NKR cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other.1 Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.1 Unlike MTS510, the binding of SA15-21 is not interrupted by LPS stimulation1. Clone SA15-21 is capable of recognizing TLR4 individually and when it is complexed with MD-2.

Additional reported applications (for the relevant formats) include: immunoprecipitation1 and inhibition of LPS-induced hepatocyte apoptosis2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Akashi S, et al. 2003. J. Exp. Med. 198:1035. (IP)
2. Wakabayashi Y, et al. 2006. J. Immunol. 177:1772. (Block)
3. Visintin A, et al. 2006. J. Leukoc. Biol. 80:1584. (FC)
4. Shibata T, et al. 2011. Int. Immunol. 23:503. (FC)

Thioglycollate-elicited C57BL/6 mouse peritoneal macrophages

Thioglycollate-elicited C57BL/6 mouse peritoneal macrophages were stained with TLR4 (clone SA15-21) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).



Description:

Toll-like receptors are highly conserved from Drosophila to humans and share structural and functional similarities. They are type I transmembrane signaling receptors which activate the innate immune system in response to pathogen invasion. So far, at least 13 TLR members have been identified. The secretory protein, MD2, associates with cell-surface bound TLR4 (CD284) on monocytes, B cells, and T cells. TLR4 has been recognized as critical for host recognition of LPS in conjunction with MD2.

Other Names: Toll-like Receptor 4/MD2 complex, TLR4-MD2-complex, TLR4/MD2, CD284/MD2
Structure: Type I transmembrane protein complex
Distribution: Macrophages, B cells and T cells
Ligand Receptor: Bacterial LPS
Interaction: Activates the innate immune system against Gram-negative pathogens by recognition of LPS
Antigen References:

1. Brennan TV, et al. 2012. Blood 120:2899.
2. Wantia N, et al. 2011. PLoS One 6:e26101.
3. Loser K, et al. 2010. Nat. Med. 16:713.
4. Kang S, et al. 2009. Blood 113:2605.