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Safety Data Sheet (SDS) PE Anti-mouse IL-33Ralpha IL1RL1 ST2 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse IL-33Rα (IL1RL1, ST2) Antibody
1326515 25 µg $125.00       
1326520 100 µg $285.00       
Clone: DIH9
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: IL-33Rα-hFcγ1 fusion protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Hashiguchi M, et al. 2014. Eur. J. Immunology. (FC) PubMed
2. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed

Mouse Th2 clone D10.G4.1 was

Mouse Th2 clone D10.G4.1 was stained with anti-mouse IL-33Ra/ST2 (clone DIH9) PE (filled histogram) or rat IgG1, κ PE isotype control (open histogram).



Description:

IL-33Rα, also known as ST2 or IL-1RL1, is a member of the Toll/IL-1 receptor family.  It binds IL-33 and is structurally similar to IL-1R1.  Two forms of the protein exist - a soluble form known as ST2 and a membrane anchored form known as ST2L.  The membrane form is expressed by Th2 cells and bone marrow derived mast cells, whereas the soluble form is expressed by serum-stimulated fibroblasts.

Blocking with anti-ST2 antibodies has been shown to alleviate experimental arthritis and airway inflammation.  The IL-33-ST2 axis has been reported to be important across a range of diseases including asthma, allergies, and cardiac disease.

Other Names: T1/ST2, ST2L, IL-1R1, T1, DER4, IL1R1
Structure: 63 kD, IL-1 receptor family member, single pass type I membrane protein
Distribution: Mast cells, Th2 cells, and fibroblasts
Function: Following interaction with IL-33, it recruits MyD88, Irak1, Irak4 and Traf6 followed by phosphorylation of the MAP/ERK pathway.
Ligand Receptor: IL-33
Interaction: Interacts with MyD88, Irak1, Irak4 and Traf6
Antigen References:

1. Yanagisawa K, et al. 1993. FEBS Lett. 318:83.
2. Schmitt E, et al. 1990. Cytokine 6:407.
3. Yanagisawa K, et al. 1992. FEBS Lett. 302:51.
4. Takagi T, et al. 1993. Biochim Biophys Acta. 1178:194.