The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
Clone L138D7 staining works optimally at room temperature or 4°C. Unlike other chemokine receptor antibodies, avoid using L138D7 at 37°C.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC and CXCR5 (clone L138D7) PE (top) or rat IgG2b, κ PE isotype control (bottom).
CD185 is also known as CXCR5. It is the receptor for chemokine CXCL13/BLC, which is chemotactic for B cells. CXCR5 is expressed on B cells and a subset of T cells in the spleen, neuronal tissue, lymph nodes, and bone marrow. It is important for migration of B cells into the B cell follicles of the spleen and Peyer’s patches. Follicular helper T cells (Tfh) also express CXCR5 and the ability of these cells to migrate to the lymph node is modulated by the balanced expression of CCR7 and CXCR5.
BLR1, GPCR6, MDR-15
42 kD, seven transmembrane protein
B cells and a subset of T cells in mouse spleen, Tfh cells, also expressed in lymph node, bone marrow, and brain
B cell migration into follicles, possible regulatory role in B cell differentiation and Burkitt's lymphoma
1. Kaiser E, et al. 1993. Eur. J. Immunol. 23:2532. 2. Forster R, et al. 1994. Cell. Mol. Biol. 40:381. 3. Forster R, et al. 1994. Blood 84:830. 4. Forster R, et al. 1996. Cell 87:1037. 5. Wolf I, et al. 1998. J. Biol. Chem. 273:28831.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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