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Safety Data Sheet (SDS) PE Anti-mouse PLZF Antibody     Product Data Sheet (PDF)    
PE Anti-mouse PLZF Antibody
1329015 25 µg $120.00       
1329020 100 µg $290.00       
Clone: 9E12
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Combination of peptides covering the amino, carboxyl and hinge regions of the PLZF.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis using 1% PFA plus 70% ethanol. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

C57BL/6 mouse leukocytes from mouse

C57BL/6 mouse leukocytes from mouse liver were surface stained with CD45 PerCP, CD8 Alexa Fluor® 488, and NK1.1 Brilliant Violet 421™. Cells were then fixed and permeabilized with 1% PFA plus 70% ethanol, followed by intracellular staining with PLZF (clone 9E12) PE or Armenian hamster IgG PE isotype control. Data shown was gated on CD45+/CD8+ positive cells.





Description:

PLZF (promyelocytic leukemia zinc finger), also known as Zbtb16, Zinc finger and BTB domain-containing protein 16, Zfp145, is a member of the BTB-POZ family of transcription factors. It was first identified in a patient with acute promyelocytic leukemia, where a reciprocal chromosomal translocation t(11;17)(q23;q21) resulted in a fusion with RARA gene encoding retinoic acid receptor alpha. Expression of this transcriptional repressor in immune cells differs between human and mouse. In humans, PLZF is expressed in CD34+ progenitor cells and in primitive multipotent hematopoietic cell lines, NK cells, γ/δ T cells, CD4+ and CD8+ T cells. It is also expressed in MR1-specific mucosal-associated invariant T cells as well as in MHC class II-restricted T cells that develop via a thymocyte-thymocyte interaction. PLZF is involved in NK cell function, cellular quiescence, and growth suppression. It also inhibits gene expression induced by retinoic acid receptor. In mice, PLZF is highly expressed in immature CD1d-resricted invariant NKT (iNKT) cells, a subset of γ/δ (Vg1.1+Vd6.3+) T cells, and non-invariant CD1d-restricted T cells. PLZF exists as a homodimer or in complex with PLZP, and has been shown to be involved in the development of NKT cells. It is also reported to be expressed in embryonic tissues, giving rise to hematopoietic progenitors.

Other Names: Promyelocytic leukemia zinc finger, Zbtb16, Zinc finger and BTB domain-containing protein 16, Zfp145
Structure: BTB domain-containing
Distribution: iNKT cells, γ/δ (Vg1.1+Vd6.3+) T cells, non-invariant CD1d-restricted T cells
Function: Involved in NKT cell development
Antigen References:

1. Constantinides MG, et al. 2011. J. Immunol. 187:309.
2. Kovalovsky D, et al. 2008. Nat. Immunol. 9:1055.
3. Chen Z, et al. 1993. EMBO J. 12:1161.
4. Chen Z, et al. 1994. Proc. Natl. Acad. Sci. USA 91:1178.